Genotyping and primer details


Genotyping and primer details for 480A12 - At3g10550
Line ID 480A12
Locus At3g10550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3294394 of line 480A12 Chr3:3294394
   
Insertion allele amplicon 8474 - 66qz
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 66qz: 5'-AAAATCTACCAGGAAAGTCTGCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3294198
   
wt amplicon (over InsPos) 01rp - 66qz
wt amplicon start Chr3:3294198
wt amplicon end Chr3:3294971
wt amplicon size 774 bp
Locus specific primer 66qz: 5'-AAAATCTACCAGGAAAGTCTGCAA-3'
2nd locus specific primer 01rp: 5'-GTATTCCCTGTGCTATTTGGTGTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11