Genotyping and primer details


Genotyping and primer details for 486B04 - At1g54220
Line ID 486B04
Locus At1g54220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:20246673 of line 486B04 Chr1:20246673
   
Insertion allele amplicon 8474 - 18de
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 18de: 5'-TTAGAAAAACCCGAAAGAAACAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20246352
   
wt amplicon (over InsPos) 59dy - 18de
wt amplicon start Chr1:20246352
wt amplicon end Chr1:20247052
wt amplicon size 701 bp
Locus specific primer 18de: 5'-TTAGAAAAACCCGAAAGAAACAGA-3'
2nd locus specific primer 59dy: 5'-ACAATTGGAGAAGAAGTTCGGTTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11