Genotyping and primer details


Genotyping and primer details for 487F04 - At3g59910
Line ID 487F04
Locus At3g59910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22131793 of line 487F04 Chr3:22131793
   
Insertion allele amplicon 8474 - 45jd
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 45jd: 5'-AAACTCTACTCCTTGGAGAGACCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22131217
   
wt amplicon (over InsPos) 07jz - 45jd
wt amplicon start Chr3:22131217
wt amplicon end Chr3:22132172
wt amplicon size 956 bp
Locus specific primer 45jd: 5'-AAACTCTACTCCTTGGAGAGACCC-3'
2nd locus specific primer 07jz: 5'-AGACAAAACAATTCCGCTTAAGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11