Genotyping and primer details


Genotyping and primer details for 492C04 - At3g06180
Line ID 492C04
Locus At3g06180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:1873071 of line 492C04 Chr3:1873071
   
Insertion allele amplicon 8474 - KG15
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KG15: 5'-GAAGATAACGGCGTAAACTGAATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:1873503
   
wt amplicon (over InsPos) 06sl - KG15
wt amplicon start Chr3:1872571
wt amplicon end Chr3:1873503
wt amplicon size 933 bp
Locus specific primer KG15: 5'-GAAGATAACGGCGTAAACTGAATC-3'
2nd locus specific primer 06sl: 5'-TTGTTTGTGATGAATGAATTGTGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11