Genotyping and primer details


Genotyping and primer details for 493G08 - At1g01570
Line ID 493G08
Locus At1g01570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:207167 of line 493G08 Chr1:207167
   
Insertion allele amplicon 8474 - 09md
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 09md: 5'-ATTTACAAGCTCGAAATTGCTGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:207526
   
wt amplicon (over InsPos) 10md - 09md
wt amplicon start Chr1:206693
wt amplicon end Chr1:207526
wt amplicon size 833 bp
Locus specific primer 09md: 5'-ATTTACAAGCTCGAAATTGCTGTT-3'
2nd locus specific primer 10md: 5'-TTGATTTATACGGGAAACTTCTGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11