Genotyping and primer details


Genotyping and primer details for 497B06 - At5g37720
Line ID 497B06
Locus At5g37720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:14982964 of line 497B06 Chr5:14982964
   
Insertion allele amplicon 8474 - JU36
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JU36: 5'-CAAAATCCCTGTTAGAAAGAGCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:14982601
   
Insertion allele amplicon 8474 - JU37
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JU37: 5'-TTGCGACAGTACTTAGAACCTGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:14983487
   
wt amplicon (over InsPos) JU37 - JU36
wt amplicon start Chr5:14982601
wt amplicon end Chr5:14983487
wt amplicon size 886 bp
Locus specific primer JU36: 5'-CAAAATCCCTGTTAGAAAGAGCAT-3'
2nd locus specific primer JU37: 5'-TTGCGACAGTACTTAGAACCTGAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11