Genotyping and primer details


Genotyping and primer details for 500A03 - At5g44490
Line ID 500A03
Locus At5g44490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:17925949 of line 500A03 Chr5:17925949
   
Insertion allele amplicon 8474 - 38tv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 38tv: 5'-GGAAAATACGATTAGGTTTCCGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:17926271
   
wt amplicon (over InsPos) 03ul - 38tv
wt amplicon start Chr5:17925450
wt amplicon end Chr5:17926271
wt amplicon size 822 bp
Locus specific primer 38tv: 5'-GGAAAATACGATTAGGTTTCCGAT-3'
2nd locus specific primer 03ul: 5'-TCTCAAGAAACTTGTTGTGCATTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11