Genotyping and primer details


Genotyping and primer details for 501A06 - At1g18720
Line ID 501A06
Locus At1g18720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6458920 of line 501A06 Chr1:6458920
   
Insertion allele amplicon 8474 - CW74
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CW74: 5'-TATCGGTTTGGATAAGAAATCTGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6459404
   
wt amplicon (over InsPos) 06rp - CW74
wt amplicon start Chr1:6458573
wt amplicon end Chr1:6459404
wt amplicon size 832 bp
Locus specific primer CW74: 5'-TATCGGTTTGGATAAGAAATCTGG-3'
2nd locus specific primer 06rp: 5'-TGGTGAGTATTAACTGTTTTCCCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11