Genotyping and primer details


Genotyping and primer details for 501B04 - At1g55440
Line ID 501B04
Locus At1g55440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:20701811 of line 501B04 Chr1:20701811
   
Insertion allele amplicon 8474 - 54wp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 54wp: 5'-TGTCTCCCACAACTTTATCACATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20701176
   
Insertion allele amplicon 8474 - AP36
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AP36: 5'-CCAAGACCTTTTCCCATACAATTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20701518
   
Insertion allele amplicon 8474 - CC72
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CC72: 5'-TGGTTGTATGGAGTGTGATTTTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20702351
   
wt amplicon (over InsPos) AP36 - CC72
wt amplicon start Chr1:20701518
wt amplicon end Chr1:20702351
wt amplicon size 834 bp
Locus specific primer CC72: 5'-TGGTTGTATGGAGTGTGATTTTGT-3'
2nd locus specific primer AP36: 5'-CCAAGACCTTTTCCCATACAATTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11