Genotyping and primer details


Genotyping and primer details for 504H09 - At5g11870
Line ID 504H09
Locus At5g11870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:3825878 of line 504H09 Chr5:3825878
   
Insertion allele amplicon 8474 - 62ra
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 62ra: 5'-CCTCGTCAACTACGACAACATTAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3825470
   
wt amplicon (over InsPos) 30rt - 62ra
wt amplicon start Chr5:3825470
wt amplicon end Chr5:3826248
wt amplicon size 779 bp
Locus specific primer 62ra: 5'-CCTCGTCAACTACGACAACATTAG-3'
2nd locus specific primer 30rt: 5'-TCAGGTTTGAGATGATAAAAAGCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11