Genotyping and primer details


Genotyping and primer details for 510A05 - At1g34050
Line ID 510A05
Locus At1g34050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:12394380 of line 510A05 Chr1:12394380
   
Insertion allele amplicon 8474 - CC78
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CC78: 5'-CTAGATGGAAGACGGTTTCTTTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:12394805
   
Insertion allele amplicon 8474 - CC79
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CC79: 5'-ATTGTGGCTCAGATATTAGCGTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:12393801
   
wt amplicon (over InsPos) CC79 - CC78
wt amplicon start Chr1:12393801
wt amplicon end Chr1:12394805
wt amplicon size 1004 bp
Locus specific primer CC78: 5'-CTAGATGGAAGACGGTTTCTTTTG-3'
2nd locus specific primer CC79: 5'-ATTGTGGCTCAGATATTAGCGTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11