Genotyping and primer details


Genotyping and primer details for 511F01 - At5g42860
Line ID 511F01
Locus At5g42860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:17183846 of line 511F01 Chr5:17183846
   
Insertion allele amplicon 8474 - AL04
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AL04: 5'-CTTGTGTTCGAAATTAATCAAGCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:17183393
   
Insertion allele amplicon 8474 - KN59
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KN59: 5'-AGATGCTGGAGGTATAGGAACTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:17184311
   
wt amplicon (over InsPos) AL04 - KN59
wt amplicon start Chr5:17183393
wt amplicon end Chr5:17184311
wt amplicon size 919 bp
Locus specific primer KN59: 5'-AGATGCTGGAGGTATAGGAACTGA-3'
2nd locus specific primer AL04: 5'-CTTGTGTTCGAAATTAATCAAGCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11