Genotyping and primer details


Genotyping and primer details for 511G12 - At1g23880
Line ID 511G12
Locus At1g23880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:8436898 of line 511G12 Chr1:8436898
   
Insertion allele amplicon 8474 - 31cp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 31cp: 5'-AAAAATCACCTGCTTCACTGATCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:8437417
   
wt amplicon (over InsPos) 10dn - 31cp
wt amplicon start Chr1:8436546
wt amplicon end Chr1:8437417
wt amplicon size 872 bp
Locus specific primer 31cp: 5'-AAAAATCACCTGCTTCACTGATCT-3'
2nd locus specific primer 10dn: 5'-GTCTCTCTCTTTCAAAACCACCAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11