Genotyping and primer details


Genotyping and primer details for 514G05 - At1g60070
Line ID 514G05
Locus At1g60070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:22145228 of line 514G05 Chr1:22145228
   
Insertion allele amplicon 8474 - 37sn
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 37sn: 5'-TCTCCTGTAAGCAATACAACAAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:22144886
   
wt amplicon (over InsPos) 35vc - 37sn
wt amplicon start Chr1:22144886
wt amplicon end Chr1:22145529
wt amplicon size 644 bp
Locus specific primer 37sn: 5'-TCTCCTGTAAGCAATACAACAAGC-3'
2nd locus specific primer 35vc: 5'-TTTGGGGATCTCTTTCTTAATTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11