Genotyping and primer details


Genotyping and primer details for 516H06 - At2g41150
Line ID 516H06
Locus At2g41150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17154211 of line 516H06 Chr2:17154211
   
Insertion allele amplicon 8474 - CC89
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CC89: 5'-CCCACGTTCTTTCAATTTCATACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17154668
   
wt amplicon (over InsPos) 30sq - CC89
wt amplicon start Chr2:17153861
wt amplicon end Chr2:17154668
wt amplicon size 808 bp
Locus specific primer CC89: 5'-CCCACGTTCTTTCAATTTCATACT-3'
2nd locus specific primer 30sq: 5'-TGAGCAAACCCCATAAGTTAAAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11