Genotyping and primer details


Genotyping and primer details for 517H08 - At4g16141
Line ID 517H08
Locus At4g16141 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:9132326 of line 517H08 Chr4:9132326
   
Insertion allele amplicon 8474 - 24uv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 24uv: 5'-TCTTTCACTCAACCATTACGACAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:9131818
   
wt amplicon (over InsPos) 57vd - 24uv
wt amplicon start Chr4:9131818
wt amplicon end Chr4:9132605
wt amplicon size 788 bp
Locus specific primer 24uv: 5'-TCTTTCACTCAACCATTACGACAC-3'
2nd locus specific primer 57vd: 5'-GTTGATCTTGTGTGCTTAGCTCTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11