Genotyping and primer details


Genotyping and primer details for 518A09 - At3g49720
Line ID 518A09
Locus At3g49720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18440624 of line 518A09 Chr3:18440624
   
Insertion allele amplicon 8474 - 30fb
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 30fb: 5'-GTGACTATTCTTGCACAGCAGAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18441115
   
wt amplicon (over InsPos) 05fz - 30fb
wt amplicon start Chr3:18440232
wt amplicon end Chr3:18441115
wt amplicon size 884 bp
Locus specific primer 30fb: 5'-GTGACTATTCTTGCACAGCAGAAG-3'
2nd locus specific primer 05fz: 5'-TTGTATAATCCTTTGGAAACAGCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11