Genotyping and primer details


Genotyping and primer details for 518D09 - At4g19460
Line ID 518D09
Locus At4g19460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:10611210 of line 518D09 Chr4:10611210
   
Insertion allele amplicon 8474 - 15jr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 15jr: 5'-TTGTAGAAACCCTTGAGCTCATTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:10610765
   
wt amplicon (over InsPos) 05kp - 15jr
wt amplicon start Chr4:10610765
wt amplicon end Chr4:10611587
wt amplicon size 823 bp
Locus specific primer 15jr: 5'-TTGTAGAAACCCTTGAGCTCATTT-3'
2nd locus specific primer 05kp: 5'-CCTTCACTCTATACACCGCCTTAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11