Genotyping and primer details


Genotyping and primer details for 521G06 - At3g57800
Line ID 521G06
Locus At3g57800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21411713 of line 521G06 Chr3:21411713
   
Insertion allele amplicon 8474 - JL40
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JL40: 5'-TTAGCACTTGAATTGGAAGGTACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21410912
   
wt amplicon (over InsPos) 18ho - JL40
wt amplicon start Chr3:21410912
wt amplicon end Chr3:21411960
wt amplicon size 1049 bp
Locus specific primer JL40: 5'-TTAGCACTTGAATTGGAAGGTACA-3'
2nd locus specific primer 18ho: 5'-AATAAATATGTGCCGTTTTCTGGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11