Genotyping and primer details


Genotyping and primer details for 526C02 - At2g20780
Line ID 526C02
Locus At2g20780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:8948729 of line 526C02 Chr2:8948729
   
Insertion allele amplicon 8474 - CD74
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CD74: 5'-ATAGTAGACTGTGGCGTCAATTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:8948111
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11