Genotyping and primer details


Genotyping and primer details for 529B11 - At1g16520
Line ID 529B11
Locus At1g16520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5650823 of line 529B11 Chr1:5650823
   
Insertion allele amplicon 8474 - 02de
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 02de: 5'-CAGCTTCAAAATACACTGGAAACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5650277
   
wt amplicon (over InsPos) 03ec - 02de
wt amplicon start Chr1:5650277
wt amplicon end Chr1:5651163
wt amplicon size 887 bp
Locus specific primer 02de: 5'-CAGCTTCAAAATACACTGGAAACA-3'
2nd locus specific primer 03ec: 5'-AGAGAAACTCAACACTTCCCAAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11