Genotyping and primer details


Genotyping and primer details for 535B06 - At4g28650
Line ID 535B06
Locus At4g28650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:14145015 of line 535B06 Chr4:14145015
   
Insertion allele amplicon 8474 - EQ92
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EQ92: 5'-GTGCTAGATCTCTCCAACAATTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14145623
   
Insertion allele amplicon 8474 - KR78
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KR78: 5'-CCACAATGTCTACTGATTCTCCAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14144445
   
wt amplicon (over InsPos) KR78 - EQ92
wt amplicon start Chr4:14144445
wt amplicon end Chr4:14145623
wt amplicon size 1179 bp
Locus specific primer EQ92: 5'-GTGCTAGATCTCTCCAACAATTCC-3'
2nd locus specific primer KR78: 5'-CCACAATGTCTACTGATTCTCCAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11