Genotyping and primer details


Genotyping and primer details for 535E08 - At3g46500
Line ID 535E08
Locus At3g46500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:17121667 of line 535E08 Chr3:17121667
   
Insertion allele amplicon 8474 - CH06
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CH06: 5'-AGACTTACGGAATATCGGTCTTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:17105151
   
wt amplicon (over InsPos) 18vg - CH06
wt amplicon start Chr3:17105151
wt amplicon end Chr3:17121241
wt amplicon size 901 bp
Locus specific primer CH06: 5'-AGACTTACGGAATATCGGTCTTGA-3'
2nd locus specific primer 18vg: 5'-GTATATACGCGTCTCATGTGTGCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11