Genotyping and primer details


Genotyping and primer details for 535E12 - At1g02750
Line ID 535E12
Locus At1g02750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:603793 of line 535E12 Chr1:603793
   
Insertion allele amplicon 8474 - 25wj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 25wj: 5'-TGACAACAGTCCCTGTACAAACTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:604095
   
wt amplicon (over InsPos) 30wt - 25wj
wt amplicon start Chr1:603049
wt amplicon end Chr1:604095
wt amplicon size 1047 bp
Locus specific primer 25wj: 5'-TGACAACAGTCCCTGTACAAACTC-3'
2nd locus specific primer 30wt: 5'-TTTGCGCAAGTGATTATGATTTAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11