Genotyping and primer details


Genotyping and primer details for 536D02 - At3g16150
Line ID 536D02
Locus At3g16150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:5472007 of line 536D02 Chr3:5472007
   
Insertion allele amplicon 8474 - 19kk
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 19kk: 5'-GAATAAATGGACGGTGGAGATTTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:5472477
   
wt amplicon (over InsPos) 03ll - 19kk
wt amplicon start Chr3:5471469
wt amplicon end Chr3:5472477
wt amplicon size 1009 bp
Locus specific primer 19kk: 5'-GAATAAATGGACGGTGGAGATTTA-3'
2nd locus specific primer 03ll: 5'-GAAAATGGTAAAACAAATCCCAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11