Genotyping and primer details


Genotyping and primer details for 545C04 - At1g17600
Line ID 545C04
Locus At1g17600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6054929 of line 545C04 Chr1:6054929
   
Insertion allele amplicon 8474 - CW76
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CW76: 5'-GATCCCGTCACATCTAGTCTCTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6054343
   
wt amplicon (over InsPos) 02lo - CW76
wt amplicon start Chr1:6054343
wt amplicon end Chr1:6055248
wt amplicon size 906 bp
Locus specific primer CW76: 5'-GATCCCGTCACATCTAGTCTCTTC-3'
2nd locus specific primer 02lo: 5'-TTGAAAGCAGTCTTGACGAGAATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11