Genotyping and primer details

Genotyping and primer details for 545D03 - At1g25510
Line ID 545D03
Locus At1g25510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:8959621 of line 545D03 Chr1:8959621
Insertion allele amplicon 8474 - 29op
Locus specific primer 29op: 5'-GTGTCTTTACGAGGTTTCTTACGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:8960164
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11