Genotyping and primer details


Genotyping and primer details for 546G06 - At4g17410
Line ID 546G06
Locus At4g17410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:9718391 of line 546G06 Chr4:9718391
   
Insertion allele amplicon 8474 - 17jr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 17jr: 5'-TCAACTGGCTTTTCCACTTTAACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:9718964
   
wt amplicon (over InsPos) 25kj - 17jr
wt amplicon start Chr4:9717993
wt amplicon end Chr4:9718964
wt amplicon size 972 bp
Locus specific primer 17jr: 5'-TCAACTGGCTTTTCCACTTTAACT-3'
2nd locus specific primer 25kj: 5'-CGGTTATTACCGAGAAATGAATGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11