Genotyping and primer details


Genotyping and primer details for 554E06 - At5g52390
Line ID 554E06
Locus At5g52390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:21264590 of line 554E06 Chr5:21264590
   
Insertion allele amplicon 8474 - JL46
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JL46: 5'-TGTAAAAGTTACACCAGAACGCAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:21264084
   
wt amplicon (over InsPos) 09id - JL46
wt amplicon start Chr5:21264084
wt amplicon end Chr5:21265153
wt amplicon size 1070 bp
Locus specific primer JL46: 5'-TGTAAAAGTTACACCAGAACGCAG-3'
2nd locus specific primer 09id: 5'-CGTAGCTTTTATGATTCTCCTTGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11