Genotyping and primer details


Genotyping and primer details for 563F02 - At1g49100
Line ID 563F02
Locus At1g49100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:18167329 of line 563F02 Chr1:18167329
   
Insertion allele amplicon 8474 - EU02
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EU02: 5'-GTTGAAGGAAACCCTAAGCTTCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:18167822
   
Insertion allele amplicon 3144 - NK68
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer NK68: 5'-GAACAATTGGTGGTCTACATCCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:18166786
   
wt amplicon (over InsPos) NK68 - EU02
wt amplicon start Chr1:18166786
wt amplicon end Chr1:18167822
wt amplicon size 1037 bp
Locus specific primer EU02: 5'-GTTGAAGGAAACCCTAAGCTTCTT-3'
2nd locus specific primer NK68: 5'-GAACAATTGGTGGTCTACATCCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11