Genotyping and primer details


Genotyping and primer details for 570B04 - At1g48540
Line ID 570B04
Locus At1g48540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:17949421 of line 570B04 Chr1:17949421
   
Insertion allele amplicon 8474 - 31uj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 31uj: 5'-GAGATAAAAATGAAGGGGACATGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:17949807
   
wt amplicon (over InsPos) 15vu - 31uj
wt amplicon start Chr1:17948788
wt amplicon end Chr1:17949807
wt amplicon size 1020 bp
Locus specific primer 31uj: 5'-GAGATAAAAATGAAGGGGACATGA-3'
2nd locus specific primer 15vu: 5'-TTCTGAGTAGCTGAGGGAAAAGAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11