Genotyping and primer details


Genotyping and primer details for 572C12 - At1g80000
Line ID 572C12
Locus At1g80000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:30096248 of line 572C12 Chr1:30096248
   
Insertion allele amplicon 8474 - 28dl
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 28dl: 5'-CCACCTTATACTGTCCTTGATGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:30095819
   
wt amplicon (over InsPos) 09ee - 28dl
wt amplicon start Chr1:30095819
wt amplicon end Chr1:30096701
wt amplicon size 883 bp
Locus specific primer 28dl: 5'-CCACCTTATACTGTCCTTGATGGT-3'
2nd locus specific primer 09ee: 5'-GTCAACATGTGCTCGTTAGTGTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11