Genotyping and primer details


Genotyping and primer details for 579D05 - At4g23470
Line ID 579D05
Locus At4g23470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:12250893 of line 579D05 Chr4:12250893
   
Insertion allele amplicon 8474 - TN05
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer TN05: 5'-CTCACAAGCAATTACAAACACGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:12250413
   
wt amplicon (over InsPos) 62dy - TN05
wt amplicon start Chr4:12250413
wt amplicon end Chr4:12251201
wt amplicon size 789 bp
Locus specific primer TN05: 5'-CTCACAAGCAATTACAAACACGAT-3'
2nd locus specific primer 62dy: 5'-CTCATACAAACAACGACCTCAAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11