Genotyping and primer details


Genotyping and primer details for 579F06 - At5g05470
Line ID 579F06
Locus At5g05470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1621313 of line 579F06 Chr5:1621313
   
Insertion allele amplicon 8474 - 40sa
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 40sa: 5'-ATAAATAGGAGCACACCCAACAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1621785
   
wt amplicon (over InsPos) 03sq - 40sa
wt amplicon start Chr5:1620810
wt amplicon end Chr5:1621785
wt amplicon size 976 bp
Locus specific primer 40sa: 5'-ATAAATAGGAGCACACCCAACAAC-3'
2nd locus specific primer 03sq: 5'-CTTCGTGTCGATAGAGAGAGAGGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11