Genotyping and primer details


Genotyping and primer details for 583E05 - At5g20540
Line ID 583E05
Locus At5g20540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:6949399 of line 583E05 Chr5:6949399
   
Insertion allele amplicon 8474 - 18lu
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 18lu: 5'-AATTTGATTACAGGACAAAGACGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:6949841
   
wt amplicon (over InsPos) 10mo - 18lu
wt amplicon start Chr5:6948903
wt amplicon end Chr5:6949841
wt amplicon size 939 bp
Locus specific primer 18lu: 5'-AATTTGATTACAGGACAAAGACGC-3'
2nd locus specific primer 10mo: 5'-AACAATCATGCCATGTCTTTTGTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11