Genotyping and primer details


Genotyping and primer details for 587H06 - At2g29390
Line ID 587H06
Locus At2g29390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:12612325 of line 587H06 Chr2:12612325
   
Insertion allele amplicon 8474 - 27nt
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 27nt: 5'-AATGAGAACACTGACAACTTGGTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:12611894
   
wt amplicon (over InsPos) 02oe - 27nt
wt amplicon start Chr2:12611894
wt amplicon end Chr2:12612945
wt amplicon size 1052 bp
Locus specific primer 27nt: 5'-AATGAGAACACTGACAACTTGGTG-3'
2nd locus specific primer 02oe: 5'-GTGTGATGGAGAGAAACAAACAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11