Genotyping and primer details


Genotyping and primer details for 588F04 - At1g07900
Line ID 588F04
Locus At1g07900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:2442967 of line 588F04 Chr1:2442967
   
Insertion allele amplicon 8474 - 42tv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 42tv: 5'-CGTAGAGAAATTTAGTAGCCACCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:2442390
   
wt amplicon (over InsPos) 22vb - 42tv
wt amplicon start Chr1:2442390
wt amplicon end Chr1:2443475
wt amplicon size 1086 bp
Locus specific primer 42tv: 5'-CGTAGAGAAATTTAGTAGCCACCG-3'
2nd locus specific primer 22vb: 5'-TTGAAATTGCATATTCACCATCTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11