Genotyping and primer details


Genotyping and primer details for 590A04 - At2g22400
Line ID 590A04
Locus At2g22400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:9506912 of line 590A04 Chr2:9506912
   
Insertion allele amplicon 8474 - 22dr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 22dr: 5'-TAATCATGCAGGTACCTCCTCTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:9507622
   
wt amplicon (over InsPos) 14ep - 22dr
wt amplicon start Chr2:9506530
wt amplicon end Chr2:9507622
wt amplicon size 1093 bp
Locus specific primer 22dr: 5'-TAATCATGCAGGTACCTCCTCTCT-3'
2nd locus specific primer 14ep: 5'-GTCTCCACACCTCCTTAGAATCTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11