Genotyping and primer details


Genotyping and primer details for 590B04 - At5g41180
Line ID 590B04
Locus At5g41180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:16484750 of line 590B04 Chr5:16484750
   
Insertion allele amplicon 3144 - 35wq
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer 35wq: 5'-GTGACTATCTCAAAATTCCGAAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:16485603
   
Insertion allele amplicon 8474 - EU11
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EU11: 5'-ATTGGAATGGCATTAAATGTTCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:16484399
   
wt amplicon (over InsPos) ST02 - EU11
wt amplicon start Chr5:16484399
wt amplicon end Chr5:16485258
wt amplicon size 860 bp
Locus specific primer EU11: 5'-ATTGGAATGGCATTAAATGTTCTC-3'
2nd locus specific primer ST02: 5'-TCTTCCCTCGAAAAAGTTGTAAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11