Genotyping and primer details


Genotyping and primer details for 592C03 - At4g29930
Line ID 592C03
Locus At4g29930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:14646808 of line 592C03 Chr4:14646808
   
Insertion allele amplicon 8474 - 28uv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 28uv: 5'-GGCAGCACGTAAAAAGAAAATTAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14647157
   
wt amplicon (over InsPos) 51vy - 28uv
wt amplicon start Chr4:14646133
wt amplicon end Chr4:14647157
wt amplicon size 1025 bp
Locus specific primer 28uv: 5'-GGCAGCACGTAAAAAGAAAATTAG-3'
2nd locus specific primer 51vy: 5'-ATTTCGCCTTGACTTTTTCTGATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11