Genotyping and primer details


Genotyping and primer details for 594D07 - At1g08590
Line ID 594D07
Locus At1g08590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:2719206 of line 594D07 Chr1:2719206
   
Insertion allele amplicon 8474 - EU14
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EU14: 5'-AATGGAACTCGCATATTATTCCAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:2718645
   
Insertion allele amplicon 8474 - EU15
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EU15: 5'-AGCCATTGTAACCAAGAATAATGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:2719555
   
wt amplicon (over InsPos) EU15 - EU14
wt amplicon start Chr1:2718645
wt amplicon end Chr1:2719555
wt amplicon size 911 bp
Locus specific primer EU14: 5'-AATGGAACTCGCATATTATTCCAC-3'
2nd locus specific primer EU15: 5'-AGCCATTGTAACCAAGAATAATGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11