Genotyping and primer details


Genotyping and primer details for 594E02 - At5g49430
Line ID 594E02
Locus At5g49430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:20038018 of line 594E02 Chr5:20038018
   
Insertion allele amplicon 8474 - 29uv
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 29uv: 5'-CTGCAGAATGAATCCATAGAACAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:20037534
   
wt amplicon (over InsPos) 14vl - 29uv
wt amplicon start Chr5:20037534
wt amplicon end Chr5:20038486
wt amplicon size 953 bp
Locus specific primer 29uv: 5'-CTGCAGAATGAATCCATAGAACAA-3'
2nd locus specific primer 14vl: 5'-CTCTTATCTGCGGCTGTTACTCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11