Genotyping and primer details


Genotyping and primer details for 595B03 - At1g10390
Line ID 595B03
Locus At1g10390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:3410069 of line 595B03 Chr1:3410069
   
Insertion allele amplicon 8474 - 66wf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 66wf: 5'-TAACTGCTAATTGGTGTGTTGCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:3410724
   
Insertion allele amplicon 8474 - CD88
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CD88: 5'-GAAGTTGTTGAAACCAAAACCATT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:3409442
   
wt amplicon (over InsPos) CD89 - CD88
wt amplicon start Chr1:3409442
wt amplicon end Chr1:3410622
wt amplicon size 1181 bp
Locus specific primer CD88: 5'-GAAGTTGTTGAAACCAAAACCATT-3'
2nd locus specific primer CD89: 5'-GTTCTGGTTTTGGTCAATCAACTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11