Genotyping and primer details


Genotyping and primer details for 604E11 - At3g05420
Line ID 604E11
Locus At3g05420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:1566319 of line 604E11 Chr3:1566319
   
Insertion allele amplicon 8474 - 18vc
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 18vc: 5'-CTGTTAATAATGCCACAACCAGAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:1565944
   
wt amplicon (over InsPos) 22vl - 18vc
wt amplicon start Chr3:1565944
wt amplicon end Chr3:1566612
wt amplicon size 669 bp
Locus specific primer 18vc: 5'-CTGTTAATAATGCCACAACCAGAG-3'
2nd locus specific primer 22vl: 5'-CAGTTTGAAACACCTTGATTGTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11