Genotyping and primer details


Genotyping and primer details for 607C02 - At3g60900
Line ID 607C02
Locus At3g60900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22500382 of line 607C02 Chr3:22500382
   
Insertion allele amplicon 8474 - 20lu
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 20lu: 5'-GCTTTCACATTATCTCTCCTCACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22500811
   
wt amplicon (over InsPos) 05mx - 20lu
wt amplicon start Chr3:22500038
wt amplicon end Chr3:22500811
wt amplicon size 774 bp
Locus specific primer 20lu: 5'-GCTTTCACATTATCTCTCCTCACC-3'
2nd locus specific primer 05mx: 5'-TAACGATCCTTTGGGTTTGTATTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11