Genotyping and primer details


Genotyping and primer details for 614B03 - At2g47610
Line ID 614B03
Locus At2g47610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:19530477 of line 614B03 Chr2:19530477
   
Insertion allele amplicon 8474 - 08ih
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 08ih: 5'-GTTGAGGCCGTATTTGACTACAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:19530850
   
wt amplicon (over InsPos) 07jf - 08ih
wt amplicon start Chr2:19529893
wt amplicon end Chr2:19530850
wt amplicon size 958 bp
Locus specific primer 08ih: 5'-GTTGAGGCCGTATTTGACTACAAT-3'
2nd locus specific primer 07jf: 5'-ATTTGGGATCTGTTAGTTGTTTGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11