Genotyping and primer details


Genotyping and primer details for 614F01 - At2g19780
Line ID 614F01
Locus At2g19780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:8523524 of line 614F01 Chr2:8523524
   
Insertion allele amplicon 8474 - 03co
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 03co: 5'-AAGAATCATGAAGAAGAAAGGCAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:8524046
   
wt amplicon (over InsPos) 06da - 03co
wt amplicon start Chr2:8523207
wt amplicon end Chr2:8524046
wt amplicon size 840 bp
Locus specific primer 03co: 5'-AAGAATCATGAAGAAGAAAGGCAC-3'
2nd locus specific primer 06da: 5'-ATGTCGGAGAATAAACCAACTGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11