Genotyping and primer details


Genotyping and primer details for 621H03 - At1g63640
Line ID 621H03
Locus At1g63640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:23594097 of line 621H03 Chr1:23594097
   
Insertion allele amplicon 8474 - CH82
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CH82: 5'-TTTCTGACTGCTATGGATGAAATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:23594399
   
wt amplicon (over InsPos) 01or - CH82
wt amplicon start Chr1:23593557
wt amplicon end Chr1:23594399
wt amplicon size 843 bp
Locus specific primer CH82: 5'-TTTCTGACTGCTATGGATGAAATG-3'
2nd locus specific primer 01or: 5'-TAGGTCAGTCTTTGGCTTACATGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11