Genotyping and primer details


Genotyping and primer details for 622B07 - At2g39960
Line ID 622B07
Locus At2g39960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:16682567 of line 622B07 Chr2:16682567
   
Insertion allele amplicon 8474 - 29vh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 29vh: 5'-GAAGCAATCCCACTAACCAAATTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:16682151
   
wt amplicon (over InsPos) 65vo - 29vh
wt amplicon start Chr2:16682151
wt amplicon end Chr2:16683142
wt amplicon size 992 bp
Locus specific primer 29vh: 5'-GAAGCAATCCCACTAACCAAATTA-3'
2nd locus specific primer 65vo: 5'-TGATACTTTCTGCTTTCTGCTTGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11