Genotyping and primer details


Genotyping and primer details for 626H08 - At1g17230
Line ID 626H08
Locus At1g17230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5893901 of line 626H08 Chr1:5893901
   
Insertion allele amplicon 8474 - EU22
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EU22: 5'-AATCATAGCGACCACTTCTCTCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5894669
   
wt amplicon (over InsPos) TH46 - EU22
wt amplicon start Chr1:5893912
wt amplicon end Chr1:5894669
wt amplicon size 758 bp
Locus specific primer EU22: 5'-AATCATAGCGACCACTTCTCTCAT-3'
2nd locus specific primer TH46: 5'-ATCAACTCTTGGAAAGATAAGGCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11